Small-Molecule Sensing: A Direct Enzyme-Linked Immunosorbent Assay for the Monosaccharide Kdo

Abstract

Captured and bound! Kdo, a monosaccharide, and an immobilized capture molecule form a covalent adduct that can be detected by adduct-specific antibodies (see scheme). The recognition yields a signal that is proportional to the amount of Kdo present in solution. This small-molecule sensor should be applicable for any small molecule that can react to give an immunogenic adduct. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.