Abstract
In North America, red blood cells (RBCs) are cryopreserved in a clinical setting using high glycerol concentrations (40% w/v) with slow cooling rates (~1°C/min) prior to storage at −80°C, while European protocols use reduced glycerol concentrations with rapid freezing rates. After thawing and prior to transfusion, glycerol must be removed to avoid intravascular hemolysis. This is a time consuming process requiring specialized equipment. Small molecule ice recrystallization inhibitors (IRIs) such as β-PMP-Glc and β-pBrPh-Glc have the ability to prevent ice recrystallization, a process that contributes to cellular injury and decreased cell viability after cryopreservation. Herein, we report that addition of 110 mM β-PMP-Glc or 30 mM β-pBrPh-Glc to a 15% glycerol solution increases post-thaw RBC integrity by 30-50% using slow cooling rates and emphasize the potential of small molecule IRIs for the preservation of cells.
Highlights
Small Molecule Ice Recrystallization Inhibitors Enable Freezing of Human Red Blood Cells with Reduced Glycerol Concentrations
Typical clinical cryopreservation protocols for red blood cells (RBCs) in Canada and the United States rely on using high amounts of glycerol (,40%) combined with a slow freezing rate (1uC/min) to 280uC1,7
Deglycerolization must be performed prior to transfusion to ensure intracellular glycerol concentrations are less than 1% to prevent intravascular hemolysis upon transfusion[5,7,8,9]
Summary
Small Molecule Ice Recrystallization Inhibitors Enable Freezing of Human Red Blood Cells with Reduced Glycerol Concentrations. Complicated and time consuming post-thaw deglycerolization procedures are necessary to ensure glycerol concentrations are reduced to less than 1% prior to transfusion to prevent post-transfusion intravascular hemolysis[5,7,8,9]. Current research on improving RBC cryopreservation has focused on a number of different aspects of the cryopreservation protocol in an attempt www.nature.com/scientificreports to overcome the limitations associated with using glycerol as a cryoprotectant Towards this end, other cryoprotectants have been examined including non-penetrating additives such as hydroxyethyl starch (HES), polyvinylpyrrolidone (PVP) and dextran[1,29,30,31,32]. The use of small molecule ice recrystallization inhibitors for the cryopreservation of human RBCs has not been explored
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
