Abstract
Chemical tools that allow the real-time monitoring of organ function and the visualisation of organ-related processes at the cellular level are of great importance in biological research. The upregulation/downregulation of specific biomarkers is often associated with the development of organ related diseases. Small-molecule fluorescent probes have the potential to create advances in our understanding of these disorders. Viable probes should be endowed with a number of key features that include high biomarker sensitivity, low limit of detection, fast response times and appropriate in vitro and in vivo biocompatibility. In this tutorial review, we discuss the development of probes that allow the targeting of organ related processes in vitro and in vivo. We highlight the design strategy that underlies the preparation of various promising probes, their optical response to key biomarkers, and proof-of-concept biological studies. The inherent drawbacks and limitations are discussed as are the current challenges and opportunities in the field. The hope is that this tutorial review will inspire the further development of small-molecule fluorescent probes that could aid the study of pathogenic conditions that contribute to organ-related diseases.
Highlights
The human body contains multiple major organs, all of which are crucial to maintaining healthy function.Each major organ plays a specific role in the body; synergy between various major organs is required for optimal health
Reaction with GSH catalysed by glutathione S-transferase p (GSTp) that is overexpressed in various tumour cells leads to formation of a covalently bound adduct that can react further with sulfhydryl groups present in a variety of tumour proteins to produce an anti-tumour effect (Fig. 31)
Monitoring the onset and progression of diseases related to major organs, as well as differentiating acute and chronic forms of the same diseases, is beneficial
Summary
The human body contains multiple major organs (cf. Scheme 1a), all of which are crucial to maintaining healthy function. His research interests in the development of new fluorescent probes for disease diagnosis and active analyte detection He Tian Jr. is a PhD student in Applied Chemistry under the supervision of Prof. Which are designed to bind/react with a target disease-based biomarker and provide a measurable change to the fluorescence signal These small molecule-based fluorescent probes should have high sensitivity and specificity for their target analyte to ensure its accurate detection within a biological system. The design of effective small molecule-based fluorescent probes for interrogating and evaluating the development of major organ disease should meet the following requirements: significant signal change before and after biomarker recognition, fluorophore with good photostability, high fluorescence quantum yield, adequate water solubility, cell permeability and acceptable biocompatibility. More than one strategy may be employed; in others, the design of effective probes remains an unmet need that would benefit from further research and development efforts
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
