Abstract
Small plants from the pea cross-inoculation group were selected for rapid nodulation on agar in test tubes. Plants from the genus Vicia were best; strains of the species hirsuta, tetrasperma, sativa and lathyroides nodulated respectively 4, 5, 4 and 7 days after inoculation with Rhizobium leguminosarum RBL 1. Rapid nodulation, visibility of the root system and in situ acetylene reduction makes the culture tube system ideal for genetic studies of Rh. leguminosarum. A number of bacteria including Agrobacterium tumefaciens containing a Sym-plasmid from Rh. leguminosarum formed root nodules. A Vicia sativa strain formed thick and short roots (Tsr) when inoculated with a bacterium harbouring a Sym-plasmid from Rh. leguminosarum. Crown gall formation could also be investigated by wounding of axenic Vicia hirsuta in test tubes.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
