Small intestinal submucosa gel as a potential scaffolding material for cardiac tissue engineering

Abstract

Cardiac tissue engineering typically utilizes protein-rich scaffolding materials and growth factors to improve cardiac tissue function in vitro and in vivo. The objectives of this preliminary study were (i) to investigate the potential of porcine small intestinal submucosa gel (SIS gel) in cardiac tissue engineering and (ii) to compare the function of tissues based on either SIS gel or Matrigel, a tumor-derived benchmark material. Neonatal rat cardiac cells were combined with either SIS gel or Matrigel and cultured on porous elastomeric scaffolds composed of poly(glycerol sebacate) for 13 days. Tissue function was assessed by measuring contraction rates twice daily. Tissue morphology was compared qualitatively by hematoxylin and eosin staining. Normalized troponin T expression (troponin T:DNA) was compared using image analysis. SIS gel constructs contracted at significantly higher rates than Matrigel constructs on days 8–11. Normalized troponin T expression was significantly higher in SIS gel constructs compared with Matrigel constructs. In summary, this research demonstrated that: (i) SIS gel can be used to create contractile engineered cardiac tissue; (ii) SIS gel produced engineered cardiac tissues with a more physiological contraction rate and higher phenotypic protein expression based on the basic in vitro examinations performed in this study.