Abstract
BackgroundPrevious research has demonstrated that ectopic expression of Ran-binding protein (RanBP) in Arabidopsis results in more axillary buds and reduced apical dominance compared to WT plants. However, the function of RanBP in poplar, which has very typical secondary growth, remains unclear. Here, the Populus deltoides (Marsh.) RanBP gene (PdRanBP) was isolated and functionally characterized by ectopic expression in a hybrid poplar (P. davidiana Dode × P. bolleana Lauche).ResultsPdRanBP was predominantly expressed in leaf buds and tissues undergoing secondary wall expansion, including immature xylem and immature phloem in the stem. Overexpression of PdRanBP in poplar increased the number of sylleptic branches and the proportion of cells in the G2 phase of the cell cycle, retarded plant growth, consistently decreased the size of the secondary xylem and secondary phloem zones, and reduced the expression levels of cell wall biosynthesis genes. The downregulation of PdRanBP facilitated secondary wall expansion and increased stem height, the sizes of the xylem and phloem zones, and the expression levels of cell wall biosynthesis genes.ConclusionsThese results suggest that PdRanBP influences the apical and radial growth of poplar trees and that PdRanBP may regulate cell division during cell cycle progression. Taken together, our results demonstrated that PdRanBP is a nuclear, vascular tissue development-associated protein in P. deltoides.Electronic supplementary materialThe online version of this article (doi:10.1186/s12863-016-0403-4) contains supplementary material, which is available to authorized users.
Highlights
Previous research has demonstrated that ectopic expression of Ran-binding protein (RanBP) in Arabidopsis results in more axillary buds and reduced apical dominance compared to WT plants
Isolation and phylogenetic analysis of Populus deltoides (Marsh.) RanBP gene (PdRanBP) A 670-bp cDNA fragment of P. deltoides RanBP was amplified by reverse transcription PCR (RT-PCR) and sequenced
In conclusion, the cloning and detailed characterization of PdRanBP from the developing xylem of poplar trees support the notion that this gene is associated with tree growth and vascular tissue development
Summary
Previous research has demonstrated that ectopic expression of Ran-binding protein (RanBP) in Arabidopsis results in more axillary buds and reduced apical dominance compared to WT plants. The Populus deltoides (Marsh.) RanBP gene (PdRanBP) was isolated and functionally characterized by ectopic expression in a hybrid poplar (P. davidiana Dode × P. bolleana Lauche). The process of wood formation and development is mediated by the activity of the vascular cambium, which is a meristematic cell population that facilitates vascular tissues development in tree stems [1]. Small GTP-binding genes play diverse roles in a multitude of cellular processes, such as microtubule organization, vesicle-mediated intracellular trafficking, signal transduction, and cell growth and division in plants and animals [10, 11]. Ran interacts with importin or exportin proteins to regulate a variety of biochemical processes, including nuclear envelope assembly, nucleo-cytoplasmic signal transfer, cell
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