Abstract
The synthesis, sorting, and characterization of monodisperse gold nanorods with dimensions around 10 nm in length and below 6 nm in diameter is reported. They display tunable plasmon resonance in the near infrared, a region where cellular absorption is reduced. A dual color photothermal microscope is developed to demonstrate that they are promising single molecule probes for bioimaging.
Highlights
To cite this version: Edakkattuparambil Sidharth Shibu, Nadezda Varkentina, Laurent Cognet, Brahim Lounis
The overall size of the functional biomarkers is a general issue for any imaging approach because of steric hindrance effects in confined cell regions
Single-molecule absorption microscopy was early used to detect single-molecules[10] at cryogenic temperatures, it is only with the advent of photothermal microscopy[11,12] that practical applications of absorption microscopy were developed in single-molecule research
Summary
To cite this version: Edakkattuparambil Sidharth Shibu, Nadezda Varkentina, Laurent Cognet, Brahim Lounis. Photothermal microscopies are totally insensitive to non-absorbing scatterers, even when large objects with strong refractive index contrasts are present within the surroundings of the imaged nanoparticles, as is often the case in biological samples.[23] Several live cell application of PhI involve the detection of small gold nanospheres in order to perform long term single molecule tracking.[9,24,25] A limitation arises due to background signal from endogenous cellular components, since these nanoparticles have to be excited at their plasmon resonance, which lies around 530 nm.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
