Abstract
The presence of polydisperse small circular DNAs in wheat cells was first confirmed by the mica-pressadsorption (MPA) method for electron microscopy. To identify their location in the cell, chloroplast and mitochondrial fractions were examined separately by the same method; small circular DNAs were scarcely found in the former but abundantly in the latter fraction, indicating their origin from mitochondria. The size varied greatly, ranging from 0.1 to 2.0 μm in contour length. To verify the present finding, the same mitochondrial fraction was examined by the conventional cytochrome-spreading method by which the presence of the same size-class of circular DNAs was confirmed.
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