Small changes in gene expression of targeted osmoregulatory genes when exposing marine and freshwater threespine stickleback (Gasterosteus aculeatus) to abrupt salinity transfers.

Annette Taugbøl,Kjartan Østbye,Leif Asbjørn Vøllestad,Tina Arntsen,Vincent Laudet

doi:10.1371/journal.pone.0106894

Annette Taugbøl, Kjartan Østbye + Show 3 more

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https://doi.org/10.1371/journal.pone.0106894

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Journal: PloS one	Publication Date: Sep 29, 2014
Citations: 83	License type: CC BY 4.0

Affiliation: University of Oslo

Abstract

Salinity is one of the key factors that affects metabolism, survival and distribution of fish species, as all fish osmoregulate and euryhaline fish maintain osmotic differences between their extracellular fluid and either freshwater or seawater. The threespine stickleback (Gasterosteus aculeatus) is a euryhaline species with populations in both marine and freshwater environments, where the physiological and genomic basis for salinity tolerance adaptation is not fully understood. Therefore, our main objective in this study was to investigate gene expression of three targeted osmoregulatory genes (Na+/K+-ATPase (ATPA13), cystic fibrosis transmembrane regulator (CFTR) and a voltage gated potassium channel gene (KCNH4) and one stress related heat shock protein gene (HSP70)) in gill tissue from marine and freshwater populations when exposed to non-native salinity for periods ranging from five minutes to three weeks. Overall, the targeted genes showed highly plastic expression profiles, in addition the expression of ATP1A3 was slightly higher in saltwater adapted fish and KCNH4 and HSP70 had slightly higher expression in freshwater. As no pronounced changes were observed in the expression profiles of the targeted genes, this indicates that the osmoregulatory apparatuses of both the marine and landlocked freshwater stickleback population have not been environmentally canalized, but are able to respond plastically to abrupt salinity challenges.

Highlights

The ability to respond rapidly to environmental change is beneficial in variable environments, making phenotypically plastic organisms better adapted in unstable and unpredictable environments [1]
Overall there was a tendency for the relative expression to increase with time, and the time-adjusted mean fold change was larger for the two control treatments (SS: 1.59260.087; from freshwater (FF): 1.42760.099) than for the transfer treatments (SF: 0.90360.088; fish transferred to saltwater (FS): 1.15560.094)
The increased expression of KCNH4 in freshwater found in this study indicates a potential regulatory effect of the inversion on chromosome 1, more studies are needed in order to disentangle the complete effect of the inversion

Summary

Introduction

The ability to respond rapidly to environmental change is beneficial in variable environments, making phenotypically plastic organisms better adapted in unstable and unpredictable environments [1]. Evolutionary theory predicts loss of plasticity after periods of environmental stability, when environmental constancy eliminates or weakens the source of selection that was formerly important for its maintenance, given that the cost for the trait is high [3], or through environmentally induced genetic assimilation [4] which reduces the environmental influence on trait expression. Phenotypic plasticity of a trait is generally assumed to be under selection when a single organism is exposed to several environments during its lifetime which each select for different trait values. Most fish species are stenohaline, living either in fresh or salt water [5,6] where they are exposed to the same type of osmoregulatory challenge during their lifetime. Even fewer can tolerate extreme changes in osmolality over short time scales, such as the killifishes (Fundulus spp.) [9] and the threespine stickleback (Gasterosteus aculeatus) [10,11]

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