Slow-wave activity preceding the onset of 10-15-Hz sleep spindles and 5-9-Hz oscillations in electroencephalograms in rats with and without absence seizures.

Abstract

Cortico-thalamocortical networks generate sleep spindles and slow waves during non-rapid eye movement sleep, as well as paroxysmal spike-wave discharges (i.e. electroencephalogram manifestation of absence epilepsy) and 5-9-Hz oscillations in genetic rat models (i.e. pro-epileptic activity). Absence epilepsy is a disorder of the thalamocortical network. We tested a hypothesis that absence epilepsy associates with changes in the slow-wave activity before the onset of sleep spindles and pro-epileptic 5-9-Hz oscillations. The study was performed in the WAG/Rij genetic rat model of absence epilepsy and Wistar rats at the age of 9-12months. Electroencephalograms were recorded with epidural electrodes from the anterior cortex. Sleep spindles (10-15Hz), 5-9-Hz oscillations and their slow-wave (2-7Hz) precursors were automatically detected and analysed using continuous wavelet transform. Subjects with electroencephalogram seizures (the "epileptic" phenotype) and without seizure activity (the "non-epileptic" phenotype) were identified in both strains. It was found that time-amplitude features of sleep spindles and 5-9-Hz oscillations were similar in both rat strains and in both phenotypes. Sleep spindles in "epileptic" rats were more often preceded by the slow-wave (~4Hz) activity than in "non-epileptic" rats. The intrinsic frequency of slow-wave precursors of sleep spindles and 5-9-Hz oscillations in "epileptic" rats was 1-1.5Hz higher than in "non-epileptic" rats. In general, our results indicated that absence epilepsy associated with: (a) the reinforcement of slow waves immediately prior to normal sleep spindles; and (b) weakening of amplitude growth in transition "slow wave → spindle/5-9-Hz oscillation".