Abstract
Objective: To clarify the possibility that midband Lp in LDL fractions might act as an atherogenic lipoprotein in their interaction with macrophages. Design and Methods: Low density lipoproteins (LDL) isolated by zonal ultracentrifugation from midband lipoprotein-positive serum in type IIb hyperlipidemics were subjected to polyacrylamide gel disc electrophoresis. Results: A part of midband lipoprotein was observed between preβ- and β-band, in addition to the main β-band. We named this midband lipoprotein “slowβ-migrating Lp (slowβ-Lp).” The larger LDL subtraction from midband-lipoprotein positive serum on Sepharose 2B column chromatography contained much slowβ-Lp, named slowβ-Lp-rich LDL. The smaller LDL subfraction contained a little slowβ-Lp, named slowβ-Lp-poor LDL. Slowβ-Lp-rich LDL had similar composition to the control LDL except for apolipoprotein E. The uptake of [ 3H]cholesteryl linoleate-labeled slowβ-Lp-rich LDL by J774 macrophages was higher than that of control LDL. The cholesterol ester content of J774 macrophages incubated with slowβ-Lp-rich LDL increased significantly compared with slowβ-Lp-poor LDL, β-VLDL, and control LDL. Conclusion: These results suggest that slowβ-Lp- in type IIb might generate foam cells from macrophages in atherosclerotic lesions.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
