Slow drift compensation in long-term measurements in fluorescence nanoscopy with blinking colloidal semiconductor quantum dots

Aleksei Baev,Aleksei Rozhentsov,A.V Naumov,M.G Gladush,K.R Karimullin

doi:10.1051/epjconf/201819004002

Aleksei Baev, Aleksei Rozhentsov + Show 3 more

Open Access

https://doi.org/10.1051/epjconf/201819004002

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Abstract

In this work we present an approach for image processing accuracy increasing in fluorescence nanoscopy of single colloidal quantum dots with blinking fluorescent behavior. The approach is based on the estimation of parameters, describing the slow collective drift, according to the information about a long series of frames with fluorescent images of single blinking quantum dots. The results of collective drift compensation for single colloidal quantum dots CdSe / ZnS supper-resolution image are demonstrated.

Highlights

The presence of a slow drift - spatial image shift in time - is one of the serious problems of various nanoscopy techniques
We propose an approach based on the use of information on the subdi-fractional coordinates of flickering quantum dots recorded in a sufficiently long series of frames that does not require special markers
The obtained functional dependence will characterize the law of global drift of all marks

Summary

Introduction

The presence of a slow drift - spatial image shift in time - is one of the serious problems of various nanoscopy techniques. In present time there exist a few approaches to compensate nanodrifts: by measuring the interframe shift based on calculating the cross-correlation function between frames [1], by using the special embedded markers [2], etc. We propose an approach based on the use of information on the subdi-fractional coordinates of flickering quantum dots recorded in a sufficiently long series of frames (from several hundred to several thousand frames) that does not require special markers.

Results

Conclusion