Slit-Scan-Durchflußzytometrie von Chromosomenaberrationen: Perspektiven in der Biologischen Dosimetrie

Abstract

In biological dosimetry, the dose can be directly estimated from radiation-induced chromosome aberrations in peripheral blood lymphocytes. A new technique called fluorescence-in-situ-hybridization allows to delineate chromosome aberrations by specific fluorescence labels. Usually, quantitative analysis is performed by visual or digital microscopy. For statistical reasons, a very high number of cells has to be analyzed. A perspective to further accelerate this procedure is slit-scan flow cytometry. Recent developments show that it is possible to analyze chromosomes in real-time so that they can be sorted afterwards. This should permit to sort only those chromosomes classified to be aberrant by preanalysis. By further, detailed microscopic evaluation the rate of false classifications may be reduced.