Abstract
The Sleeping Beauty (SB) transposon insertional mutagenesis system offers a streamlined approach to identify genetic drivers of cancer. With a relatively random insertion profile, SB is uniquely positioned for conducting unbiased forward genetic screens. Indeed, SB mouse models of cancer have revealed insights into the genetics of tumorigenesis. In this review, we highlight experiments that have exploited the SB system to interrogate the genetics of cancer in distinct biological contexts. We also propose experimental designs that could further our understanding of the relationship between tumor microenvironment and tumor progression.
Highlights
We propose experimental designs that could further our understanding of the relationship between tumor microenvironment and tumor progression
Sleeping Beauty (SB) is a two-part DNA transposon system that has become an integral tool in identifying genetic drivers of cancers in mouse models
SB-induced cancer models have led to the discovery of novel cancer genes while comparative genomics have demonstrated the relevance of these models to human disease [1,2,3,4,5,6,7,8]
Summary
Mouse Models of Cancer: Microenvironmental Influences on Cancer Genetics. The Sleeping Beauty (SB) transposon insertional mutagenesis system offers a streamlined approach to identify genetic drivers of cancer. With a relatively random insertion profile, SB is uniquely positioned for conducting unbiased forward genetic screens. SB mouse models of cancer have revealed insights into the genetics of tumorigenesis. We highlight experiments that have exploited the SB system to interrogate the genetics of cancer in distinct biological contexts. We propose experimental designs that could further our understanding of the relationship between tumor microenvironment and tumor progression
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