Abstract
The solute carrier (SLC) family-38 of transporters has eleven members known to transport amino acids, with glutamine being a common substrate for ten of them, with SLC38A9 being the exception. In this study, we examine the subcellular localization of SNAT10 in several independent immortalized cell lines and stem cell-derived neurons. Co-localization studies confirmed the SNAT10 was specifically localized to secretory organelles. SNAT10 is expressed in both excitatory and inhibitory neurons in the mouse brain, predominantly in the endoplasmic reticulum, and in the Golgi apparatus. Knock-down experiments of SNAT10, using Slc38a10-specific siRNA in PC12 cells reduced nascent protein synthesis by more than 40%, suggesting that SNAT10 might play a role in signaling pathways that regulate protein synthesis, and may act as a transceptor in a similar fashion to what has been shown previously for SLC38A2 (SNAT2) and SNAT9(SLC38A9).
Highlights
Solute carriers (SLCs) are the largest group of transporters, with 460 members [1] (Perland and Fredriksson, 2017), (Kandasamy et al 2018) divided into 52 families [2]
Using double immunocytochemistry (ICC) (Figure 1A–C) and Proximity ligation assay (PLA) (Figure 2E–G) we show that SNAT10 immunoreactivity is found in both excitatory and inhibitory neurons, at intracellular membranes close to the nuclei
To investigate the subcellular localization of SNAT10 we performed double ICC, with the KDEL (ER marker) and Golgi 58k (Golgi Marker) on cells transfected with a plasmid containing human SLC38A10 tagged with enhanced green fluorescent protein (eGFP) and FLAG epitope
Summary
Solute carriers (SLCs) are the largest group of transporters, with 460 members [1] (Perland and Fredriksson, 2017), (Kandasamy et al 2018) divided into 52 families [2]. Eleven members of the solute carrier family 38 (SLC38) proteins are encoded by genes Slc38a1-11. The family is termed SNAT or sodium-coupled neutral amino acid transporters, number 1–11. This family of transporters is conserved in all mammals investigated [12] and the general substrate profile for most of the SLC38s are relatively similar based on the uptake data from heterologous expression systems [8,13,14,15,16,17,18,19,20]. SNAT3 is abundant in the brain, liver, kidney, the eye, skeletal muscle, and adipose tissue [23,26,27]. SNAT1 and SNAT2 have been shown to have specific patterns of expression in the CNS by in situ hybridization and immunocytochemical studies [20,30,31]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
