SlAREB1 transcriptional activation of NOR is involved in abscisic acid-modulated ethylene biosynthesis during tomato fruit ripening

Abstract

Many studies have shown that abscisic acid (ABA) regulates climacteric fruits ripening by inducing ethylene production. Nevertheless, the key components involved in the crosstalk between these two phytohormones in controlling fruit ripening remain unknown. SlAREB1, a downstream transcription factor in ABA signaling pathway, has been reported to mediate ABA signaling that regulates tomato ripening through induction of ethylene biosynthetic genes. NOR, a member of NAC domain family, was proved to act upstream of ethylene and essential for ripening- and ethylene-associated genes expression. Here, we found that the expression of SlAREB1 and NOR are both ABA-inducible, and SlAREB1 transcription reaches the peak level prior to NOR during the ripening process. Yeast one-hybrid (Y1H), electrophoretic mobility shift assay (EMSA) and dual luciferase assay indicated NOR as a novel direct target of SlAREB1. Transient over-expression of SlAREB1 in tomato fruits results in elevated expression of NOR as well as a number of downstream ethylene biosynthetic genes including SlACS2, SlACS4 and SlACO1, suggesting that SlAREB1 can mediate ABA signal to activate NOR transcription and ultimately promote ethylene synthesis. Based on these data, we present a model suggesting that the SlAREB1-NOR regulation is a crucial node modulating ABA-regulated ethylene biosynthesis during tomato fruit ripening.