SLAM family member 8 is expressed in and enhances the growth of anaplastic large cell lymphoma

Akihiko Sugimoto,Yasuyuki Otsuka,Akifumi Takaori-Kondo,Chiyuki Ueshima,Kyohei Kitamura,Narumi Saito,Momoko Nishikori,Hironori Haga,Hiroaki Ito,Koki Moriyoshi,Yusuke Takei,Masahiro Hirata,Tatsuki R Kataoka

doi:10.1038/s41598-020-59530-1

Akihiko Sugimoto, Yasuyuki Otsuka + Show 11 more

Open Access

https://doi.org/10.1038/s41598-020-59530-1

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Journal: Scientific Reports	Publication Date: Feb 13, 2020
Citations: 13	License type: open-access

Affiliation: Kyoto University Hospital, Kyoto Medical Center

Abstract

Signaling lymphocytic activation molecule family member 8 (SLAMF8) / B-lymphocyte activator macrophage expressed/CD353 is a member of the CD2 family. SLAMF8 suppresses macrophage function but enhances the growth of neoplastic mast cells via SHP-2. In this study, we found that some anaplastic large cell lymphoma (ALCL) samples were immunohistochemically positive for SLAMF8. However, we found no significant differences between SLAMF8-positive and SLAMF8-negative ALCL samples with respect to age, gender, site, or prognosis. We also identified SLAMF8 expression in ALCL cell lines, Karpas299, and SU-DHL-1. SLAMF8 knockdown decreased the activation of SHP-2 and the growth of these cell lines, and increased the apoptosis of these cell lines. In addition, we observed the interaction between SLAMF8 and SHP-2 in these cell lines using the DuoLink in situ kit. Taken together, these results suggest that SLAMF8 may enhance the growth of ALCL via SHP-2 interaction.

Highlights

Signaling lymphocytic activation molecule family member 8 (SLAMF8) / B-lymphocyte activator macrophage expressed/CD353 is a member of the CD2 family
signaling lymphocytic activation molecule (SLAM) family signaling is generally mediated by SLAM-associated protein (SAP) or Ewing’s sarcoma-associated transcript 2 (EAT-2) in lymphocytes and macrophage cells[1,2], SLAMF8 signaling is mediated by the Src homology region 2 domain-containing phosphatase-2 (SHP-2) in human mast cells[7]
SLAMF8 suppresses the functions of human macrophages[5,6] but has an enhancing effect in human anaplastic lymphoma kinase (ALK)-positive anaplastic large cell lymphoma (ALCL) cell lines and in human KIT-mutated neoplastic mast cell lines[7]

Summary

Introduction

Signaling lymphocytic activation molecule family member 8 (SLAMF8) / B-lymphocyte activator macrophage expressed/CD353 is a member of the CD2 family. SLAMF8 suppresses macrophage function but enhances the growth of neoplastic mast cells via SHP-2. We found that some anaplastic large cell lymphoma (ALCL) samples were immunohistochemically positive for SLAMF8. We observed the interaction between SLAMF8 and SHP-2 in these cell lines using the DuoLink in situ kit. SLAM family signaling is generally mediated by SLAM-associated protein (SAP) or Ewing’s sarcoma-associated transcript 2 (EAT-2) in lymphocytes and macrophage cells[1,2], SLAMF8 signaling is mediated by the Src homology region 2 domain-containing phosphatase-2 (SHP-2) in human mast cells[7]. We analyzed the expression and function of SLAMF8 in ALCL clinical samples and cell lines

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