Skin Wound Healing Potential and Mechanisms of the Hydroalcoholic Extract of Leaves and Oleoresin of Copaifera langsdorffii Desf. Kuntze in Rats.

Lucas Fernando Sérgio Gushiken,Cláudia Helena Pellizzon,Ana Júlia Vieira,Fernando Pereira Beserra,Ariane Leite Rozza,Jairo Kenupp Bastos,Emanuel Ricardo Monteiro Martinez,Rafael Henrique Nóbrega,Marivane Lemos,Jonas Joaquim Mangabeira Da Silva,Carlos Alberto Hussni,Maurilio Polizello Junior,Carlos Roberto Padovani

doi:10.1155/2017/6589270

Lucas Fernando Sérgio Gushiken, Cláudia Helena Pellizzon + Show 11 more

Open Access

https://doi.org/10.1155/2017/6589270

Copy DOI

Abstract

The wound healing is a complex process which, sometimes, can be a problem in public health because of the possibility of physical disability or even death. Due to the lack of a gold standard drug in skin wound treatment and aiming at the discovery of new treatments in skin repair and the mechanisms involved in the process, we used oleoresin (OR) from Copaifera langsdorffii and hydroalcoholic extract of the leaves (EH) to treat rat skin wounds. For that, male Wistar rats were divided into groups (n = 8): Lanette, Collagenase, 10% EH, or 10% OR and, after anesthesia, one wound of 2 cm was made in the back of animals. The wounds were treated once a day for 3, 7, or 14 days and the wound areas were measured. The rats were euthanized and skin samples destined to biochemical, molecular, and immunohistochemical analysis. The results showed a macroscopic retraction of the wounds of 10% EH and 10% OR creams and both treatments showed anti-inflammatory activity. Molecular and immunohistochemical results demonstrated the activity of Copaifera langsdorffii creams in angiogenesis, reepithelialization, wound retraction, and remodeling mechanisms.

Highlights

Since the XVI century, the oleoresin of Copaifera langsdorffii has been used by Brazilian Indians to treat some diseases [2] and the popular knowledge reports the use of the plant as anti-inflammatory and antimicrobial agent and to treat skin wounds [1, 3]
There are some works [1, 13,14,15,16,17] which studied the wound healing potential of Copaifera langsdorffii through macroscopic analysis and collagen synthesis mechanisms, but there is the necessity of more researches about the wound healing mechanisms of the Evidence-Based Complementary and Alternative Medicine plant
As well as other plants whose wound healing potential derived from popular knowledge has been proved experimentally [29,30,31,32,33], this article proved the effectiveness of Copaifera langsdorffii creams in rat skin wound healing, according to macroscopic retraction of wounds after 14 days, besides the effect of leaves extract and oleoresin in the antiinflammatory activity, boost in reepithelialization, angiogenesis, cell proliferation, and extracellular matrix remodeling

Summary

Introduction

Since the XVI century, the oleoresin of Copaifera langsdorffii has been used by Brazilian Indians to treat some diseases [2] and the popular knowledge reports the use of the plant as anti-inflammatory and antimicrobial agent and to treat skin wounds [1, 3]. Among the biological properties of copaiba, some have already been studied such as anti-inflammatory agent [4], antinociceptive [5], antioxidant [6], gastrointestinal diseases [6,7,8,9], pulmonary and urinary disorders [10], urolithiasis [11], and analgesic agent [1, 12]. The aromatic and phenolic compounds presented in aerial parts of copaiba are important in cosmetic and pharmaceutical industries [19], besides the use of hydroalcoholic extract from the leaves to treat urolithiasis [11]

Methods

Results

Discussion

Conclusion