Abstract
Abstract Background and objectives: The ex vivo magnetic resonance microimaging (MRM) image characteristics are reported in human skin excised samples. Design and methods:Human excised skin samples were imaged using custom coil placed inside 500 MHz NMR imager for high resolution microimaging. Skin in vivo- and ex vivo MRI images were processed for characterization of different skin structures. Contiguous cross-sectional T1-weighted 3D spin echo MRI, T2-weighted 3D spin echo MRI and proton density images were compared with skin histopathology and NMR peaks. In all skin specimens, epidermis and dermis thickening and hair follicle size was measured using MRM. Results: Optimized TE and TR parameters and multicontrast enhancement generated better MRI visibility of different skin components. Within high MR signal regions near to custom coil, MRI images at short echo time were comparable with digitized histological sections for skin structures of epidermis, dermis, hair follicles in 6 (67%) of the 9 specimens from different age groups. Skin % tissue composition, measurement of epidermis, dermis, sebaceous gland and hair follicle size, skin NMR peaks were signatures of skin type. The image processing determined the dimensionality of skin tissue components and skin typing in different age groups. Conclusion: The ex vivo MRI images and histopathology of skin may measure the skin structures and skin NMR peaks with image processing may be tool for determining skin age typing and skin composition.
Highlights
Human skin tissue microimaging evaluation was reported first time by Bittoun et al 1990 using one-axis planar gradient insert to achieve high resolution in perpendicular direction to skin surface with voxel size 18x312x1000 micrometers for the identification of sebaceous glands, smooth epidermis and continuous dermis with distinct hair follicles wrapped up with epidermis layers [1]
Within high MR signal regions near to custom coil, MRI images at short echo time were comparable with digitized histological sections for skin structures of epidermis, dermis, hair follicles in 6 (67%) of the 9 specimens from different age groups
The ex vivo MRI images and histopathology of skin may measure the skin structures and skin NMR peaks with image processing may be tool for determining skin age typing and skin composition
Summary
Human skin tissue microimaging evaluation was reported first time by Bittoun et al 1990 using one-axis planar gradient insert to achieve high resolution in perpendicular direction to skin surface with voxel size 18x312x1000 micrometers for the identification of sebaceous glands, smooth epidermis and continuous dermis with distinct hair follicles wrapped up with epidermis layers [1]. To evaluate human skin samples by 500 MHz MRI visible skin features of epidermis-dermis and hair follicle features at optimized MRI scan parameters with enhanced multi-contrast using fast 3D variable flip angle gradient and multislice multiecho pulse sequences in less time; 2. To compare the MRI visible skin features in different skin age groups with morphometric histology features; and NMR peaks; and 4. Within high MR signal regions near to custom coil, MRI images at short echo time were comparable with digitized histological sections for skin structures of epidermis, dermis, hair follicles in 6 (67%) of the 9 specimens from different age groups. Skin % tissue composition, measurement of epidermis, dermis, sebaceous gland and hair follicle size, skin NMR peaks were signatures of skin type. Conclusion: The ex vivo MRI images and histopathology of skin may measure the skin structures and skin NMR peaks with image processing may be tool for determining skin age typing and skin composition
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