Abstract
Fish skin mucus serves as a first line of defense against pathogens and external stressors. In this study the proteomic profile of lumpsucker skin mucus was characterized using 2D gels coupled with tandem mass spectrometry. Mucosal proteins were identified by homology searches across the databases SwissProt, NCBInr and vertebrate EST. The identified proteins were clustered into ten groups based on their gene ontology biological process in PANTHER (www.patherdb.org). Calmodulin, cystatin-B, histone H2B, peroxiredoxin1, apolipoprotein A1, natterin-2, 14-3-3 protein, alfa enolase, pentraxin, warm temperature acclimation 65kDa (WAP65kDa) and heat shock proteins were identified. Several of the proteins are known to be involved in immune and/or stress responses. Proteomic profile established in this study could be a benchmark for differential proteomics studies.
Highlights
Cyclopterus lumpus L., commonly known as lumpsucker/lumpfish, is a semi-pelagic fish distributed throughout the North Atlantic Ocean
Skin mucus of fish contains a variety of immune relevant factors including lectins, lysozymes, calmodulin, immunoglobulins, complement, C-reactive proteins, proteolytic enzymes, anti-microbial peptides and proteins [4]
There are various techniques for mapping the proteome, classical 2D gels still have their place in the field of protein and molecular biology
Summary
Cyclopterus lumpus L., commonly known as lumpsucker/lumpfish, is a semi-pelagic fish distributed throughout the North Atlantic Ocean This fish has been valued for its roe in fish food industry for decades [1]. The skin mucus has a very important role in maintaining fish health, especially in intensive farming where level of stress and infections could be high. Skin mucus of fish contains a variety of immune relevant factors including lectins, lysozymes, calmodulin, immunoglobulins, complement, C-reactive proteins, proteolytic enzymes, anti-microbial peptides and proteins [4]. These factors form a biochemical barrier that serves as first line of defense against a wide range of pathogens. These results could be useful for implementation of health and stress management strategies for production of a more robust fish
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