Abstract

BackgroundImage processing techniques have been widely used in the analysis of leaf characteristics. Earlier techniques for processing digital RGB color images of plant leaves had several drawbacks, such as inadequate de-noising, and adopting normal-probability statistical estimation models which have few parameters and limited applicability.ResultsWe confirmed the skewness distribution characteristics of the red, green, blue and grayscale channels of the images of tobacco leaves. Twenty skewed-distribution parameters were computed including the mean, median, mode, skewness, and kurtosis. We used the mean parameter to establish a stepwise regression model that is similar to earlier models. Other models based on the median and the skewness parameters led to accurate RGB-based description and prediction, as well as better fitting of the SPAD value. More parameters improved the accuracy of RGB model description and prediction, and extended its application range. Indeed, the skewed-distribution parameters can describe changes of the leaf color depth and homogeneity.ConclusionsThe color histogram of the blade images follows a skewed distribution, whose parameters greatly enrich the RGB model and can describe changes in leaf color depth and homogeneity.

Highlights

  • Image processing techniques have been widely used in the analysis of leaf characteristics

  • We found that the tobacco leaves gradually decayed, and that the leaf color changed from green to yellow after 40 days

  • Correlation between skewed‐distribution parameters and SPAD values We have shown that the leaf RGB color distribution is a skewed distribution

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Summary

Introduction

Image processing techniques have been widely used in the analysis of leaf characteristics. Earlier techniques for processing digital RGB color images of plant leaves had several drawbacks, such as inadequate de-noising, and adopting normal-probability statistical estimation models which have few parameters and limited applicability. For an RGB color image, three color sensors per pixel can be used to capture the intensity of light in the red, green, and blue channels, respectively [11]. Existing software tools, such as MATLAB is used to process the obtained digital pictures [12]. The RGB color information of plant leaves has been exploited for the determination of chlorophyll content and indicators of changes in this content [14]. This information scarcity has become a bottleneck in the application of RGB models, greatly limiting their use

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