İskemik Kolon Anastomoz İyileşmesinde Timokinon, Zeolit ve Plateletten Zengin Plazmanın Etkinliği

Abstract

Objective: Anastomosis on ischemic intestines is a problematic issue in daily surgical routine. In this study, we investigated the potential effects of thymoquinone, zeolite and platelet rich plasma.Material and Methods: Fifty rats were divided into five groups. In the sham group all rats underwent colonic anastomosis without any ischemic insult. Colonic anastomosis was performed after mesenteric ischemia was induced by clamping the superior mesenteric artery in other study groups. In the control group, no medication was given to the rats after ishemic anastomosis. In Zeolite group and thymoquinone group, Zeolite and thymoquinone were applied locally on the anastomosis. In the platelet rich plasma group, platelet rich plasma was applied locally on the anastomosis. All the rats were sacrified on the 10th day. Blood and tissue samples were retrieved.Results: Hydroxyproline levels were not different between the groups. Bursting pressure was higher in the thymoquinone group than the control group. Reepithelization was higher than the zeolite group in the sham and thymoquinone groups. TNF-α and IL 1β levels were significantly higher in the sham group than all the other groups. TNF-α and IL 1β levels were also significantly higher in the thymoquinone, zeolite and platelet rich plasma groups than the control group and higher in the thymoquinone group than the zeolite and platelet rich plasma groups. Inflammation and ischemic necrosis levels were not different between the groups.Conclusion: Our data is suggesting that thymoquinone and platelet rich plasma have positive effects on ischemic anastomosis healing, while thymoquinone has the best results.