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Abstract
Fifteen new phage λ vectors are discussed: λSK4, λSK6, λSK10, λSK16, λSK17, λSK20, λSK21, λSK22, λSK23, λSK24, λSK25, λSK27, λSK28, λSK40 and λSK41. Their structural and functional features facilitate the implementation of a number of new strategies and cloning procedures which simplify, economize and vastly improve the utility and efficiency of library construction in λ vectors. Such improved strategies and examples of their application, with particular reference to jumping and linking libraries, are presented.
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