Abstract
Background So far, most of studies on nanometer-sized metal particles have focused on biological safety and potential hazards. However, anti-oxidative activity of noble metal nanoparticles (NPs) attracts much attention, recently. Platinum nanoparticles (Pt NPs) are one of the most important noble metals in nanotechnology because Pt NPs have negative surface potential from negative charges and are stably suspended from an electric repulsion between the same charged particles [1]. We previously reported that Pt NPs of 2-3 nm sizes scavenged reactive oxygen species (ROS) such as superoxide anion radical, hydrogen peroxide and hydroxyl radical in vitro [2]. Here, we report the cytotoxicity and size-dependent antioxidative activity of Pt NPs on rat skeletal muscle cell line, L6.
Highlights
Most of studies on nanometer-sized metal particles have focused on biological safety and potential hazards
To find the toxic effect of Platinum nanoparticles (Pt NPs) rat myoblast L6 cells were pre-cultured for 24 hours in culture medium with a 10-3 to 10 mg/l of Pt NPs and cell viability was determined by WST-1 assay
To investigate the anti-oxidative effect of Pt NPs on L6 cells, the relative amount of intracellular H2O2 was measured with a Bes-H2O2-AC florescent probe, which is designed to detect intracellular H2O2 [3]
Summary
Most of studies on nanometer-sized metal particles have focused on biological safety and potential hazards. Particle size and concentrations of Pt NPs were determined by a transmittance electron microscope (TEM) and ICP-MS, respectively. To find the toxic effect of Pt NPs rat myoblast L6 cells were pre-cultured for 24 hours in culture medium with a 10-3 to 10 mg/l of Pt NPs and cell viability was determined by WST-1 assay.
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