Siwu Granules and Erythropoietin Synergistically Ameliorated Anemia in Adenine-Induced Chronic Renal Failure Rats.

Yansheng Wu,Jiaoying Ou,Fei He,Yingqiao Li,Liqiang Shi,Jiandong Gao,Huiling Wang,Qiang Wan

doi:10.1155/2019/5832105

Abstract

Objective Renal anemia in patients with end-stage chronic kidney disease is closely related to the deterioration of cardiac function, renal function, and quality of life. This study involved adenine-induced renal anemic rat models and evaluated the treatment effect of Siwu granules and/or erythropoietin (EPO). Methods Fifty SD rats were randomly divided into 5 groups: control, model, Siwu, EPO, and Siwu plus EPO groups. The expression levels of NO, MDA, SOD, CAT, IL-6, TNF-α, EPO, EPOR, α-SMA, and TGF-β1 were detected in rats after 8 weeks of treatment with Siwu granules and/or EPO. Results After modeling, 47 rats entered the stage of treatment. Siwu plus EPO treatment significantly increased the rat hemoglobin content (p < 0.05) and reduced blood urea nitrogen (p < 0.05) and serum creatinine (p < 0.001). Compared with the control group, the expression of EPO and EPOR in the kidney of rats with renal failure was significantly decreased (p < 0.05). Moreover, the Siwu plus EPO group improved the level of oxidative stress in rats with chronic renal failure and reduced the expression of inflammatory factors. The expression of α-SMA and TGF-β1 in rats with renal failure was higher, but there was no expression in the control group. Conclusion Combined treatment of Siwu granules with EPO increased the expression of EPO and EPOR in the renal tissues and inhibited oxidative stress and inflammatory factors, improving the renal function and anemia.

Highlights

Anemia is an early sign and a common and severe complication of chronic kidney disease (CKD), especially in the end-stage renal disease (ESRD)
We used recombinant human erythropoietin (rhEPO) combined with Siwu granules to treat anemia in adenineinduced chronic renal failure rats to achieve the best therapeutic effect of kidney protection. rough protecting kidney injury, anemia caused by renal failure can be directly treated. erefore, this study focuses on the role of Siwu and EPO in protecting the kidneys, which can ameliorate renal anemia
We examined the levels of nitric oxide (NO), malondialdehyde (MDA), catalase (CAT), and superoxide dismutase (SOD) in serum and renal tissues of rats with adenine-induced chronic renal failure

Summary

Introduction

Anemia is an early sign and a common and severe complication of chronic kidney disease (CKD), especially in the end-stage renal disease (ESRD). Oxidative stress weakens the redox reaction and shortens the life of red blood cells (RBCs), resulting in the increased production of hepcidin, reduced intestinal absorption of iron, blocked mobilization of iron storage, and decreased induction of iron transporter expression and erythropoietin resistance [5]. Expert opinion recommended that while rhEPO is presently an important mainstay erythropoiesis-stimulating agent, acting as EPOR (erythropoietin receptor) agonists, and/or selectly act through downstream EPOR pathways to bolster erythroid cell formation. Such agents could lessen rhEPO dosing, side effects, and/or costs [8]. Siwu decoction increases the differentiation and proliferation of bone marrow hematopoietic stem cells and thymocytes [11, 12]

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Results

Conclusion