Sites of Glycosylation Observed by Postembedding and Preembedding Lectin Staining .

Abstract

Sites of glycosylation were investigated using postembedding and preembedding lectin staining. Positive labeling in the luminal side of perinuclear spaces and endoplasmic reticulum was observed by N-acetylgalactosamine and mannose binding lectins. Cis lamellae of the Golgi apparatus were stained by N-acetylgalactosamine and mannose binding lectins. Medial to trans lamellae were mainly labeled by galactose binding lectin and transmost lamellae were labeled by sialic acid and fucose binding lectins. The present results elucidated the route of glycoconjugate synthesis in relation to cell organelle.