Abstract
A kinetic analysis of the effects of energy poisons on β-galactoside influxes into Escherichia coli cells has been directed toward locating the site of energy coupling in the carrier mechanism for β-galactoside transport. Cyanide inhibited the influx of thiomethylgalactoside but not nitrophenylgalactoside. The inhibition of nitrophenyl-galactoside influx by thiomethylgalactoside was not affected by cyanide, and nitrophenylgalactoside also failed to relieve the cyanide inhibition of thiomethylgalactoside influx. On the basis of the steady-state kinetic predictions for a monovalent carrier mechanism, these observations have been interpreted as pointing toward the intracellular dissociation of the carrier-galactoside complex as an important site of energy coupling.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
