Abstract
TrpBA-encoded tryptophan synthetase(TSase) and aroG-encoded 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase(DAHPS) are key enzymes in tryptophan pathway in Escherichia coli. In order to improve bio-production of tryptophan through bioengineering means, a feedback inhibition resistant aroGf br(C632T) gene was cloned by SOE-PCR and co-expressed with trpBA gene. The recombinant plasmids pEtac-aroGf br-tac-trpBA and pEtac-aroG-tac-trpBA were constructed successfully in the recombinant strains, with the enzyme activity analysis indicating an increase of specific activities of DAHPS by 4.3 folds and 3.8 folds, respectively. The enzyme activity of DAHPS after mutation was increased by 1.13folds. The yield of tryptophan biosynthesis in recombinant strain E. coli JM109/pEtac-aroGfbr-tac-trpBA was increased by 1.4folds compared with that of the host strains E. coli JM109/pEtac-aroG-tac-trpBA.
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