Abstract
A procedure to produce an exact chromosomal replica of an insertion or insertion–deletion mutation produced in vitro in a plasmid with a ColE 1 origin of replication is presented. This procedure uses a previously described property of recD mutations (Biek DP, Cohen SN. J. Bacteriol. 1986;167:594–603) and is limited by (1) the compatibility of the new mutation with recD; and (2) the presence of some Escherichia coli DNA flanking the mutation.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
