Abstract
THE disaccharide trehalose was first identified as an important constituent of insect haemolymph by Wyatt and Kalf1 using silkmoth pupae, and it has since been found in other insect species and in nematodes2. Howden and Kilby3 showed that trehalose may occur in concentrations up to 2 per cent in the haemolymph of the desert locust, Schistocerca gregaria Forsk., and isolated a crystalline sample from this source. Work with the migratory locust, Locusta migratoria 4, and the blowfly, Phormia regina 5, has shown that the concentration of trehalose falls sharply during flight, and it therefore appears that this sugar is an important soluble carbohydrate and energy reserve in insects. Treherne6 has shown that radioactive glucose introduced into the gut of Schistocerca rapidly gives rise to the appearance of radioactive trehalose in the haemolymph, but nothing has been known hitherto as to the site or mode of formation of trehalose from glucose.
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