Abstract
The effects of laser light on the cellular DNA have not been extensively characterized. Low-power laser sources, such as the helium–neon (He–Ne) laser with a wavelength of 632.8 nm, have been found to produce photobiological and photodamage effects with evidence of interference with cell replication. We have investigated the effects of He–Ne laser irradiation on sister chromatid exchange (SCE) frequencies in sheep peripheral blood mononuclear cells (PBMC). Cultured cells were irradiated once at 6 selected energy intensities of laser irradiation and then stimulated with pokeweed mitogen and cultured in the presence of 5-bromodeoxyuridine (BrdUrd). The frequency of SCEs of both irradiated and non-irradiated cells were analyzed. The mean SCE of irradiated cells significantly increased with growing energy density up to a laser dose of 24 J/cm 2, whereas after an energy density of 24 J/cm 2, the SCE frequency decreased with increasing energy densities. We concluded that the application of He–Ne laser irradiation at energy densities ranging from 2 to 96 J/cm 2 produced a different effect on SCE frequency in sheep PBMC in vitro.
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