Abstract
SIRT4 belongs to the sirtuin family, which comprises NAD+ dependent lysine decaylases. SIRT4 has ADP‐ribosyltransferase, lipoamidase and decaylase activeties. In the study, we observed differential regulated lysine malonylationin ovarian granulosa cell lines (KGN) by the lable‐free quantitive technique. In SIRT4 depleted cells, there were a total of 65 malonylationsites from 56 proteins, including 39 up‐regulated malonylated proteins and 17 down‐regulated proteins. The differential regulated malonylation proteins were enriched in metabolic process and response to stimulus in GO biological process. We also detected the malonylation levels of PGK1 K131 and ENO1 K80 sites were decreased significantly in SIRT4 depleted cell lines, which were the same sites of PGK1 and ENO1 changed in PCOS primary ovarian granulosa cells (GCs). In addition, SIRT4 was decreased in PCOS GCs. In a conclusion, SIRT4 may regulate mitochondrial metabolic ability in PCOS GCs through modifying lysine malonylation of proteins in glycolysis pathway.Support or Funding InformationThis work was supported in part by the National Key R&D Program of China (2017YFC1001003, 2018YFC1003203), the National Natural Science Funds for general program (81571400, 81771580).
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