SiRNA-mediated silencing of VEGFR-3 reduces proliferation, adhesion and migration of colorectal cancer cells

Abstract

AIM: To investigate the effect of transfection with an adenovirus vector expressing short interfering RNA (siRNA) targeting vascular endothelial growth factor receptor-3 (VEGFR-3) on cell proliferation, adhesion and migration in colorectal cancer cell line LoVo. METHODS: An adenovirus vector expressing siRNA targeting VEGFR-3 was constructed and transfected into LoVo cells. The expression of VEGFR-3 was detected by RT-PCR and Western blot. Cell proliferation and migration were detected by MTT assay and Transwell assay, respectively. RESULTS: The expression of VEGFR-3 mRNA and protein was significantly decreased after transfection with the recombinant adenovirus (P < 0.05), compared with the blank control group and negative control group. The proliferation, adhesion and migration of LoVo cells were significantly decreased after transfection with the recombinant adenovirus (P < 0.05). CONCLUSION: SiRNA-mediated silencing of VEGFR-3 inhibits the proliferation, adhesion and migration of LoVo cells. © 2014 Baishideng Publishing Group Inc. All rights reserved.