Abstract
Uniform silica nanoparticles were synthesized via St(o|¨)ber method,and fluorescent europium complex with three chelate ligands(i.e.dibenzoylmethane,phenanthroline and acrylic acid)has been prepared.The hydrosoluble silica nanoparticles adsorbed liposoluble europium chelate with strong fluorescence in the aid of ultrasonication and phase-transfer solvent,and then were coated by cationic polyelectrolyte polydi-allyldimethylammonium chloride and anionic polyelectrolyte poly(acrylic acid)(PAA)one layer by one layer. The obtained fluorescent nanoparticles were charactered by transmission electron microscopy,ultraviolet spectrophotometer, infrared spectrophotometer,fluorescence spectrophotometer and Zeta potential.These nanoparticles have the advantages of uniform size,strong fluorescence,good photostability and leakage free. Futhermore,the nanoparticles with rich carboxy groups resulting from PAA coated on their surfaces made the surface modification and bioconjugation easier.These properties suggest their great potential in the application field of biology and medicine.
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