Abstract
The present study involves the development of nanobiosensor to determine toxicological behavior of Mitoxantrone (MTX). Mitoxantrone intercalates with DNA and produces MTX–DNA adduct, resulting in blockade of protein synthesis and excessive production of free radicals in the myocardium eventually leads to cardiac toxicity. Potentiometry was applied to develop an electroanalytical procedure for the determination of MTX and its interaction with DNA immobilized on the electrode surface modified with Silicon dioxide (SiO2) nanoparticles. The nanobiosensor immersed in MTX solution to monitor MTX–DNA interaction with respect to time and alters the resistance of the nanobiosensor. It was observed that MTX–DNA interaction is fast initially and as time elapses, the change in interaction gets slow due to formation of MTX–DNA adduct. Determination limit of the nanobiosensor is 100–10 ng/ml. This study suggests that the nanobiosensor allows real-time monitoring of the drug–DNA interaction changes by measuring the potential at sensor interface which can prove to be an important tool in drug discovery pipelines and molecular toxicology.
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