Sinosporangium album gen. nov., sp. nov., a new member of the suborder Streptosporangineae

Abstract

A Gram-positive, aerobic, non-motile actinobacterium, designated strain 6014(T), was isolated from a soil sample collected from Qinghai province, north-west China, and subjected to a polyphasic taxonomic study. The isolate formed elementary branching hyphae and abundant aerial mycelia with globose sporangia on ISP 4 and R2A media. Whole-cell hydrolysates of strain 6014(T) contained arabinose, galactose and ribose as diagnostic sugars and meso-diaminopimelic acid as the diagnostic diamino acid. The polar lipids consisted of phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, N-acetylglucosamine-containing phospholipids, two unknown phospholipids and an unknown glycolipid. The menaquinone system contained MK-9(H₂) and MK-9(H₄). The major fatty acids were C₁₄:₀, i-C₁₅:₀, C₁₆:₀ and 10-methyl-C₁₆:₁. The genomic DNA G+C content of the isolate was 69.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 6014(T) fell within the radius of the suborder Streptosporangineae, in which the strain formed a distinct lineage next to genera of the family Streptosporangiaceae. Based on data from this polyphasic study, strain 6014(T) can be readily distinguished from previously described organisms and represents a member of a novel species within a new genus in the suborder Streptosporangineae. The name Sinosporangium album gen. nov., sp. nov. is proposed with 6014(T) (=DSM 45181(T) =KCTC 19655(T)) as the type strain.