Single-particle orientation microscopy with quantum dots for bioimaging

Abstract

Orientational imaging of fluorescent probes has seen increasing use as a tool to study a variety of biological features, such as revealing the alignment of scaffolding and structural components in cells, or observing rotational dynamics of individual biomolecules. For high-throughput measurements, wide-field camera-based orientation imaging is the optimal approach for observing the large volumes of cells. These methods measure the polarization state of probe fluorescence to determine orientation.