Abstract
The dynamic organization of cell surface receptors plays an important role in signaling. Here we investigated the spatiotemporal organization of VEGF receptor-2 (VEGFR-2), a critical pro-angiogenic receptor tyrosine kinase in live, microvascular endothelial cells. Our study strategy combined live-cell single-molecule imaging of endogenous VEGFR-2 with computational image analysis and multiscale data analysis. We found that VEGFR-2, even in its basal, unstimulated state, possesses diffusion heterogeneity, with a mobile subpopulation and a subpopulation of restricted mobility, and assembly state heterogeneity, where receptors can be organized as monomeric or non-monomeric.
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