Abstract
Crimean-Congo hemorrhagic fever virus (CCHFV) is an emerging tick-borne virus from the family Nairoviridae that frequently causes lethal disease in humans. CCHFV has a wide geographic distribution,...
Highlights
Crimean-Congo hemorrhagic fever virus (CCHFV) is an emerging tick-borne virus from the family Nairoviridae that frequently causes lethal disease in humans
We hypothesized that immunization with virus-like replicon particle (VRP), that closely mimic the structure and composition of authentic CCHFV, might induce a more robust development of CCHFV immunity than current experimental vaccine options
The method for producing VRPs is based on the reverse genetics system previously described for CCHFV strain IbAr10200 [7,8]
Summary
Crimean-Congo hemorrhagic fever virus (CCHFV) is an emerging tick-borne virus from the family Nairoviridae that frequently causes lethal disease in humans. To confirm that VRPs are limited to a single replication cycle in the absence of GPC trans-complementation, we passaged VRP-ZsGreen in cells transfected with pCAGGS-GPC-Oman or an empty vector, and monitored fluorescence (Figure 1 (b)). No increase in the number of ZsGreen-positive cells was seen in the transfection control on days 2 and 3, supporting that GPC trans-complementation is required for VRP spread.
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