Abstract

A simple, rapid, and sensitive single-sweep polarographic method has been developed for investigation of concanavalin A (con A) and its interaction with selected polysaccharides. In a solution containing 0.001 m 2,2′-bipyridine, 0.015 m hexamethylenetetramine, and 0.1 m sodium chloride, con A exhibits a single-sweep polarographic wave, and the cathodic peak potential is −1.50 V (vs SCE). The peak current varies linearly with con A concentration over a range of 1.0 × 10 −8 to 1.2 × 10 −7 m by derivative single sweep polarography. A preliminary discussion on properties of the con A polarographic wave has been made. In addition, it has been demonstrated that single sweep polarography can be a useful method for studies on interactions of con A with its complementary polysaccharides in solution.

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