Abstract

It has been clear for some time that there are restrictions on the type of polynucleotide chain that can serve as a template for in vitro protein synthesis. Thus, naturally occurring messenger RNA, virus RNA, or synthetic polynucleotides will stimulate amino acid incorporation in an Escherichia coli cell-free system, while ribosomal RNA and soluble RNA are very poor templates. Likewise, single-stranded DNA is inactive, even though one of the two strands has a base sequence identical to that of the messenger RNA which is an active template both in vivo and in vitro. Although the basis of these restrictions remains obscure, some exceptions have been reported. In particular denatured DNA isolated from HeLa cells grown in tissue culture shows substantial activity in stimulating amino acid incorporation (Holland and McCarthy, 1964). Acquisition of this activity by the DNA is a direct result of the exposure of such cells to streptomycinoid antibiotics

Full Text
Paper version not known

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.