Abstract
The bulk DNA in bleomycin-sensitive ascites hepatoma, AH66, was more susceptible to single strand scission by this antibiotic than that in the resistant AH66F cells. The break was repairable in the resistant cells, while it was incompletely repaired in the sensitive cells. Rejoining was inhibited by actinomycin D but not by cycloheximide. The sedimentation coefficient of bulk DNA was reduced with increasing lysis time of the cells in alkali-sodium dodecyl sulfate before sucrose gradient centrifugation, while that of damaged DNA in bleomycin-treated cells did not change further under the same conditions. This suggests induction of breakage by the antibiotic of an alkali-labile linkage in the chromosomal DNA.
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