Abstract
Pulsed electric fields (PEF) can induce reversible or irreversible electroporation effects on bacterial cells resulting in sublethally or lethally injured cells. Hereafter, an alternative single-staining flow cytometry approach with SYTOX™ Green (SYTOX) is proposed, allowing for a straightforward and rapid screening of electroporation effects. SYTOX-staining indicated 38–63% of E. coli cells with intermediate cellular changes, likely being sublethally injured, at electric field intensities of 8 kV/cm–18 kV/cm. Compared to staining with propidium iodide, SYTOX showed a brighter fluorescence intensity, allowing for easier differentiation of subpopulations. The proportion of intermediate injury observed with SYTOX was distinctly higher than the sublethal damage detected with conventional plating on selective media (31–45%). It is, however, challenging to compare selective plating with the single-staining method based on membrane integrity, suggesting different analytical methods to indicate complex cellular states and sublethal effects.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
