Abstract
In infectious disease diagnosis prior to treatment, identification of the causative agent plays a crucial role. Polymerase chain reaction (PCR) has paved a molecular way for specific species identification. In this study, we have attempted to design a single common primer to identify the causative agent for shigellosis - Shigella spp. Bioinformatic tools like CLUSTALX for multiple sequence alignment, Primer3 for primer designing, NetPrimer for primer analysis, in silico PCR amplification tool for amplification were used.In silico PCR amplification gave the desired amplicon with the same sequence that had been used for primer designing. A single primer was thus designed for the genus Shigellaand is predicted to be a prototype sequence for easy and rapid detection of Shigella spp. Key words: Virulence factor data base (VFDB), CLUSTALX, Primer3, NetPrimer, in silicopolymerase chain reaction (PCR) amplification.
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