Abstract
Single K+ channels were studied using the patch-clamp method. A potential-dependent K+ channel of large conductance (about 100 pS at 100 mM of KCl on both membrane sides) was detected. Some properties of the channel (current-voltage relations, kinetic parameters, etc.) are presented. The channel was found to have about 16 resolvable quantized conductance substates. The data are confirmed by spontaneous channel degradation, i.e., spontaneous splitting of the channel conductance into independent conductance oligomers Some properties of the conductance oligomers of different order are described. The degree of potential dependency of the conductance oligomer parameters is a function of the number of subunits constituting the oligomer, i.e., the larger the number of subunits the more the degree of potential dependency. The data obtained are in agreement with a hypothesis that the channels studied are clusters (aggregates) of elementary channel subunits.
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