Abstract
With electron multiplier phototubes selected for low dark noise and with direct light coupling, chemiluminescent reactions in solution emitting as little as 200 photons s −1 will produce noise-equivalent counting rates. With this extremely sensitive system it is possible to observe the adventitious chemical production of excited states during the course of oxidase reactions in low intensity chemiluminescence as a tracer for specific metabolic reaction rates. The microsomal metabolism of carcinogenic poly cyclic aromatic hydrocarbons and the bactericidal action of phagocytic cells are accompanied by significant chemiluminescence. In the former, the chemiluminescence emission spectrum has been measured and the mechanism appears to proceed through a dioxetane intermediate to a diradical exciplex. The Luminol chemiluminescent reaction is proposed for the absolute calibration of single photon detectors and for specifying the intrinsic sensitivity of spectrometers. Procedures for these calibrations will be described.
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