Single-phase and two-phase cultivations using different light regimes to improve production of valuable substances in the anoxygenic photosynthetic bacterium Rhodopseudomonas faecalis PA2

Abstract

This study aimed to improve biomass, carotenoid, bacteriochlorophyll, protein, lipid, and carbohydrate contents of Rhodopseudomonas faecalis PA2 using different light regimes. Light intensity (4000, 6000, 8000, and 10,000 lx), together with photoperiod (24:0, 16:8, 12:12, and 8:16 h light/dark), was assigned as single-phase (SP) cultivation while two-phase (TP) cultivation used two light intensities (using 4000 lx as the first phase), together with the control of phase shift (3, 6, and 9 days) and photoperiod. Biomass, carotenoid, and bacteriochlorophyll contents were maximized by SP cultivation; light at 8000 lx with light-dark cycle of 24:0 was optimal for pigments synthesis. In contrast, TP was useful to enhance storage compounds; protein, lipid, and carbohydrate productivities were significantly increased by 121.69%, 101.69%, and 92.44%, respectively, in TP when compared with SP. This indicates that the novel light strategy proposed in this study was able to manipulate the production of valuable substances in this strain.