Single nucleotide polymorphisms predisposing to asthma in children of Mauritian Indian and Chinese Han ethnicity.

K Ramphul,L Hua,Q.H Liu,J Lv,D.Z Fang,R.X Ji,Y.X Bao

doi:10.1590/1414-431x20143751

K Ramphul, L Hua + Show 5 more

Open Access

https://doi.org/10.1590/1414-431x20143751

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Abstract

Our objective was to investigate the distributions of six single nucleotide polymorphisms (SNPs) MS4A2 E237G, MS4A2 C-109T, ADRB2 R16G, IL4RA I75V, IL4 C-590T, and IL13 C1923T in Mauritian Indian and Chinese Han children with asthma. This case-control association study enrolled 382 unrelated Mauritian Indian children, 193 with asthma and 189 healthy controls, and 384 unrelated Chinese Han children, 192 with asthma and 192 healthy controls. The SNP loci were genotyped using polymerase chain reaction (PCR)-restriction fragment length polymorphism for the Chinese Han samples and TaqMan real-time quantitative PCR for the Mauritian Indian samples. In the Mauritian Indian children, there was a significant difference in the distribution of IL13 C1923T between the asthma and control groups (P=0.033). The frequency of IL13 C1923T T/T in the Mauritian Indian asthma group was significantly higher than in the control group [odds ratio (OR)=2.119, 95% confidence interval=1.048-4.285]. The Chinese Han children with asthma had significantly higher frequencies of MS4A2 C-109T T/T (OR=1.961, P=0.001) and ADRB2 R16G A/A (OR=2.575, P=0.000) than the control group. The IL13 C1923T locus predisposed to asthma in Mauritian Indian children, which represents an ethnic difference from the Chinese Han population. The MS4A2 C-109T T/T and ADRB2 R16G A/A genotypes were associated with asthma in the Chinese Han children.

Highlights

Asthma, one of the most common chronic respiratory diseases of childhood, is characterized by reversible airflow obstruction due to chronic inflammation of the airways [1]
Our objective was to investigate the distributions of six single nucleotide polymorphisms (SNPs) MS4A2 E237G, MS4A2 C109T, ADRB2 R16G, IL-4 receptor alpha chain (IL4RA) I75V, IL4 C-590T, and IL13 C1923T in Mauritian Indian and Chinese Han children with asthma
In the Mauritian Indian children, there was a significant difference in the distribution of IL13 C1923T between the asthma and control groups (P=0.033)

Summary

Introduction

One of the most common chronic respiratory diseases of childhood, is characterized by reversible airflow obstruction due to chronic inflammation of the airways [1]. Elevated levels of total immunoglobulin (Ig)E and allergy-specific IgE are hallmarks of allergic inflammation [5]. Many genetic studies have shown that the C-109T polymorphism of MS4A2 encoding the b chain of the highaffinity IgE receptor is associated with increased plasma IgE levels [6] and the release of proinflammatory factors in asthmatic airways [7,8]. ADRB2 R16G, a polymorphism of the b2adrenergic receptor gene, may be strongly associated with airway hyperresponsiveness following activation by b2-adrenoceptor agonists [11]. Association of the IL-4 and IL-4 receptor alpha chain (IL4RA) gene with asthma has been reported in some studies [12,13]

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