Single Nucleotide Polymorphism (SNPs) of DNA repair genes (hMLH1 and hMSH2) in Rheumatoid arthritis Patients and its association with rheumatoid arthritis by Smoking status

Abstract

The purpose of this study is to examine the risk factors and the single nucleotide polymorphisms(SNPs) of DNA repair gene (hMLH1 and hMSH2) associated with rheumatoid arthritis by smoking status and environmental tobacco smoke(ETS). This study was planned to detect the susceptibility of the patients diagnosed by rheumatoid arthritis to the single nucleotide polymorphisms (SNPs) of DNA repair gene(hMLH1 and hMSH2) by smoking status and environmental tobacco smoke(ETS). The single nucleotide polymorphisms(SNPs) of DNA repair gene(hMLH1 and hMSH2) were analyzed by Dynamic allele specific hybridization(DASH) technique in rheumatoid arthritis patients and controls. The single nucleotide polymorphisms(SNPs) of DNA repair gene(hMLH1 and hMSH2) of patients were compared to those of age and sex-matched controls, which are statistically analyzed and adjusted by age, sex, smoking status, and ETS. This study showed that; The SNP of DNA repair gene hMLH1 was significantly associated with the susceptibility of rheumatoid arthritis, but that of hMSH2 was not. Differential effect of passive smoking on the association between the SNP of DNA repair gene hMSH2(CC type or CT type) and rheumatoid arthritis risk was found. It is suggested that the SNPs of DNA repair gene correlated with susceptibility to rheumatoid arthritis by smoking status was able to be used as its susceptibility marker and further as basic data to prevent the risk factors or rheumatoid arthritis. But the larger sample studies, including a variety of SNPs of DNA repair gene will be needed.