Abstract
In this paper, a microelectrode array DNA chip was fabricated on a glass slide using photolithography technology. Several probe DNAs with mercaptohexyl moiety at their 5′ end were immobilized on the gold electrodes by a DNA arrayer utilizing the affinity between gold and sulfur. Then target DNAs were hybridized and reacted with Hoechst 33258, which is a DNA minor groove binder and electrochemically active dye. Cyclic voltammetry in a 5 mM ferricyanide/ferrocyanide solution at 100 mV s–1 confirmed the immobilization of probe DNA on the gold electrodes. Linear sweep voltammetry or cyclic voltammetry showed a difference between target DNA and control DNA in the anodic peak current values. This was derived from Hoechst 33258 concentrated at the electrode surface through association with formed hybrids. It is suggested that this DNA chip could recognize sequence specific genes. It is also suggested that a multichannel electrochemical DNA microarray is useful to develop a portable device for a clinical gene diagnostic system.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
